| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.060456 |
| Chromosome: | chromosome_1 |
| Location: | 4322391 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g029250 | AAH1 | Aromatic amino acid hydroxylase-related protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCTACGGCGAGCTGGCGCACATGGCCTCGG |
| Internal bar code: | GATGGACTGTGGAAAGGGGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 784 |
| LEAP-Seq percent confirming: | 99.5322 |
| LEAP-Seq n confirming: | 2766 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGATCTCAGGTGCGTAGC |
| Suggested primer 2: | CACCCATGCAGCAGACTAGA |