| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.060476 |
| Chromosome: | chromosome 12 |
| Location: | 6444494 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g538000 | EZY5 | Early zygote expressed protein; (1 of 26) IPR001202 - WW domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAGTGTGGGCCTCTAGTGTAGTATCCAC |
| Internal bar code: | GGTTACAAACCGGACAAATCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1112 |
| LEAP-Seq percent confirming: | 99.6646 |
| LEAP-Seq n confirming: | 7725 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGTAGCAAGCAAAAATTG |
| Suggested primer 2: | GTCGCGCACACCTTTAAAAT |