| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.060509 |
| Chromosome: | chromosome 9 |
| Location: | 4325045 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394880 | (1 of 1) K16810 - TBCC domain-containing protein 1 (TBCCD1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGTCGTGGCGCCCCGGGGTTGGTTACCT |
| Internal bar code: | CTGGCCCACCATTTGGATCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 182 |
| LEAP-Seq percent confirming: | 99.7283 |
| LEAP-Seq n confirming: | 367 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTGGTTGTCCCTAGCAG |
| Suggested primer 2: | ACACACACACACACACGCAC |