| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.060517 |
| Chromosome: | chromosome 1 |
| Location: | 5385381 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g037900 | (1 of 2) K03257 - translation initiation factor 4A (EIF4A) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCGGTGGGCAGGGCGGTGGGATGTTGCA |
| Internal bar code: | AACTACTGCTAAACGAATGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 633 |
| LEAP-Seq percent confirming: | 99.5763 |
| LEAP-Seq n confirming: | 10576 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACGTCTGCCTGAAGGTGT |
| Suggested primer 2: | GTCTGGCAGCGAAAGAAGTC |