| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.060553 |
| Chromosome: | chromosome 9 |
| Location: | 5616055 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400960 | (1 of 1) IPR006311//IPR007657 - Twin-arginine translocation pathway, signal sequence // Glycosyltransferase AER61, uncharacterised | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGCCTGCAGCTGCTCTATGGGCGCGCCC |
| Internal bar code: | CGTCTCCGGCGGATTAAGACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 46 |
| LEAP-Seq percent confirming: | 54.2373 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTTCTGCGTTCTGTGTTA |
| Suggested primer 2: | TTGCTGAGGTGTGTTTTTCG |