| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.060608 |
| Chromosome: | chromosome 16 |
| Location: | 4737513 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686800 | PTA2 | (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain; Proton/phosphate symporter, splice variant b | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGCCCAGGTGCACAGGAACGCTCAGTTT |
| Internal bar code: | GTTGAGTGTAGGGGACAAGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 526 |
| LEAP-Seq percent confirming: | 99.645 |
| LEAP-Seq n confirming: | 3649 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTAGCAACTCCGCCTGAA |
| Suggested primer 2: | ATAAATACTACGCGGCGGTG |