| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.060613 |
| Chromosome: | chromosome 13 |
| Location: | 2137353 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g578012 | (1 of 1) PTHR11061//PTHR11061:SF29 - RNA M5U METHYLTRANSFERASE FAMILY // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCTGGGAAAAGGAGGACGGCTTGAGGTT |
| Internal bar code: | TGGTGATGTTGTGGCAGCGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 420 |
| LEAP-Seq percent confirming: | 79.3896 |
| LEAP-Seq n confirming: | 2107 |
| LEAP-Seq n nonconfirming: | 547 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGGGTGCGACGAGAGTAA |
| Suggested primer 2: | ACACACACACCTGCTCCGT |