| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.060650 |
| Chromosome: | chromosome 1 |
| Location: | 1761423 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g009450 | DNJ22 | DnaJ-like protein; (1 of 5) IPR000104//IPR001623 - Antifreeze protein, type I // DnaJ domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGCACGCACGCACTTCTACTCTCCATGC |
| Internal bar code: | GCAGTCTTGTCTTCTTAAAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1292 |
| LEAP-Seq percent confirming: | 82.6816 |
| LEAP-Seq n confirming: | 148 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGATGGCTGTGATGGTTT |
| Suggested primer 2: | CGTACCGTATGGTGTGCAAG |