Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.060660 |
Chromosome: | chromosome_10 |
Location: | 2300601 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g434600 | FAP148 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCCTGCTGCTCAACATGCTGATTGCGCTGA |
Internal bar code: | TAATTGGGTAGAATCTAAGGTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 0 |
LEAP-Seq percent confirming: | 0.174216 |
LEAP-Seq n confirming: | 2 |
LEAP-Seq n nonconfirming: | 1146 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCATCATCAAGCACAAGTGG |
Suggested primer 2: | CCTCCATCTCCTTGAGCTTG |