Insertion junction: LMJ.RY0402.060679_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.060679
Chromosome:chromosome 3
Location:5585133
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g186750 (1 of 7) IPR011989//IPR016024 - Armadillo-like helical // Armadillo-type fold CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGTGGGGCCGGTCTGTCCCATCTCGACA
Internal bar code:TCCCACGTGGCGCTCATCAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:511
LEAP-Seq percent confirming:99.4891
LEAP-Seq n confirming:779
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ACGTGTGGAAGACACGTTTG
Suggested primer 2:GACGTTGTCCGACATCACAC