| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.060701 |
| Chromosome: | chromosome 5 |
| Location: | 988677 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246752 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGATCATGAAGCCGTTTGGCTTTGGAT |
| Internal bar code: | CGAACAGGACCCACTACACGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 625 |
| LEAP-Seq percent confirming: | 95.6565 |
| LEAP-Seq n confirming: | 969 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAAACCTCTGCTTGTCTC |
| Suggested primer 2: | TGCGAGAACAAATTGCTGAC |