| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.060712 |
| Chromosome: | chromosome 1 |
| Location: | 5500041 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038800 | MIP3 | (1 of 1) K09875 - aquaporin SIP (SIP); Major intrinsic protein superfamily | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAAAGGAGCTGCGGTGACGCAGGTAGAA |
| Internal bar code: | GCATGCGTCCGTCTTGTGAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 445 |
| LEAP-Seq percent confirming: | 99.4898 |
| LEAP-Seq n confirming: | 195 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTACCCACCGCTATTTCCC |
| Suggested primer 2: | GGTTGACAACGCTTCCATTT |