| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.060734 |
| Chromosome: | chromosome 6 |
| Location: | 6304605 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g291600 | ETK1 | (1 of 1) 2.7.1.82 - Ethanolamine kinase; Ethanolamine kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGCTTGAGTTTGTGATTTTGGCCGCTG |
| Internal bar code: | AGATTATCGGCTAGACTGTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 601 |
| LEAP-Seq percent confirming: | 99.7404 |
| LEAP-Seq n confirming: | 1921 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCATGTGGACTCTAGACA |
| Suggested primer 2: | TCCCCTGGTTTGAGGTAGTG |