| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.060739 |
| Chromosome: | chromosome 12 |
| Location: | 5635545 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532250 | (1 of 12) IPR000595//IPR018490 - Cyclic nucleotide-binding domain // Cyclic nucleotide-binding-like | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACTTCCGACGTCACCCGACCCGCACCCT |
| Internal bar code: | GGGTCCCGTATTCAGGGCCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 274 |
| LEAP-Seq percent confirming: | 99.2417 |
| LEAP-Seq n confirming: | 9423 |
| LEAP-Seq n nonconfirming: | 72 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCATCCACCTCCACAACT |
| Suggested primer 2: | GGCATGTACAGCAGCGTTTA |