| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.060744 |
| Chromosome: | chromosome 9 |
| Location: | 6790036 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409500 | RPP25 | Ribonuclease MRP subunit P25; (1 of 2) PF01918 - Alba (Alba) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGACTGACGCCGGCAGAGCGCGCGCTCC |
| Internal bar code: | GCTATGAACAGACCCTATCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 789 |
| LEAP-Seq percent confirming: | 99.8012 |
| LEAP-Seq n confirming: | 9537 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTCGTAGGAGGGGTGTGG |
| Suggested primer 2: | ATGGGACAGTGGTAAGGCAG |