Insertion junction: LMJ.RY0402.060815_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.060815
Chromosome:chromosome 3
Location:507491
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g145207 CPLD33 Conserved in the Plant Lineage and Diatoms; (1 of 1) PTHR36359:SF1 - RESISTANCE TO PHYTOPHTHORA 1 PROTEIN CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCATCTGGCCCACCGACGAGCCCAGCATC
Internal bar code:GTATCCGGGCCGTGTCTGCCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:492
LEAP-Seq percent confirming:98.88
LEAP-Seq n confirming:3708
LEAP-Seq n nonconfirming:42
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGCACTCGAGGATCCAGC
Suggested primer 2:GAAGCTCTTCCACAGGAACG