Insertion junction: LMJ.RY0402.060815_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.060815
Chromosome:chromosome 3
Location:507501
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g145207 CPLD33 Conserved in the Plant Lineage and Diatoms; (1 of 1) PTHR36359:SF1 - RESISTANCE TO PHYTOPHTHORA 1 PROTEIN CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCAGGCCGCCCGCCACTAGGCAAATCCAC
Internal bar code:CCAGACCCGATAAGTGCCAATC

Confirmation - LEAP-Seq

LEAP-Seq distance:659
LEAP-Seq percent confirming:99.7135
LEAP-Seq n confirming:4525
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGCACTCGAGGATCCAGC
Suggested primer 2:GAAGCTCTTCCACAGGAACG