| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.060831 |
| Chromosome: | chromosome 8 |
| Location: | 1126695 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g362600 | BLA1 | (1 of 2) PF00144 - Beta-lactamase (Beta-lactamase); beta-lactamase family protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTAATCCTGCTTGGAAGATGAGCACATCA |
| Internal bar code: | GTGTGCCAGATCGGGAGTCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 71 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTATGCATGCGTGTGACC |
| Suggested primer 2: | CACATACAGTGTAACGGCGG |