| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.060833 |
| Chromosome: | chromosome 17 |
| Location: | 2095876 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g712250 | (1 of 4) 2.7.11.13 - Protein kinase C / PKC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGAGTGAAGGAACGGAGTATGCGTGCAA |
| Internal bar code: | TGATGGGCTGAATTTAATTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 745 |
| LEAP-Seq percent confirming: | 99.5471 |
| LEAP-Seq n confirming: | 3077 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGAACCCTCTCCAGTTGT |
| Suggested primer 2: | CCTTCAACGAGCAGACTGTG |