Insertion junction: LMJ.RY0402.060878_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.060878
Chromosome:chromosome 6
Location:7913406
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g303300 CYN37 (1 of 1) PTHR11071:SF174 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE CYP37, CHLOROPLASTIC; Cyclophilin 37 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGGGGTGACAGGTTTCTGGCTGGCCGGC
Internal bar code:AAAAGCTGGAATCGGGTCGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:570
LEAP-Seq percent confirming:84.1026
LEAP-Seq n confirming:328
LEAP-Seq n nonconfirming:62
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCGAGGTAAAGAAGATCC
Suggested primer 2:CACACTCGTGTAGTTCGCGT