| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.060886 |
| Chromosome: | chromosome 14 |
| Location: | 3390111 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630301 | (1 of 1) IPR000104//IPR001890//IPR029058 - Antifreeze protein, type I // RNA-binding, CRM domain // Alpha/Beta hydrolase fold | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACAAAACAAACAGCTGTGCAAAATACCC |
| Internal bar code: | CGCGAACATCTAAAAAATAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 259 |
| LEAP-Seq percent confirming: | 99.4732 |
| LEAP-Seq n confirming: | 1133 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGAGATCGTGTGTGGGAC |
| Suggested primer 2: | GCCACTTGATCACTCCGTTT |