| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.060890 |
| Chromosome: | chromosome 12 |
| Location: | 6915231 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g560650 | (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGACACCACCGGCACCGGAACCAGCAGTG |
| Internal bar code: | GATTCGCGAGCTTCGACACGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 512 |
| LEAP-Seq percent confirming: | 86.662 |
| LEAP-Seq n confirming: | 76591 |
| LEAP-Seq n nonconfirming: | 11788 |
| LEAP-Seq n unique pos: | 144 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAACTGCCGGGTACTGGTC |
| Suggested primer 2: | GTGATGTGTGCAGCCTGACT |