| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.060927 |
| Chromosome: | chromosome 12 |
| Location: | 2790505 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g502800 | (1 of 1) K14768 - U3 small nucleolar RNA-associated protein 7 (UTP7, WDR46) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTCGGCTTCCGGTCCTCGATGATGTTGC |
| Internal bar code: | TGACGCGGTAACGCACCTGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 753 |
| LEAP-Seq percent confirming: | 99.7815 |
| LEAP-Seq n confirming: | 9589 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGACCCTGCCTTCTCTTG |
| Suggested primer 2: | CGTCAGTGAAGACACTCCGA |