Insertion junction: LMJ.RY0402.060943_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.060943
Chromosome:chromosome 17
Location:4207261
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g730250 PIT,PIT1 (1 of 1) PTHR12175//PTHR12175:SF2 - AD039 HT014 THIOREDOXIN FAMILY TRP26 // SUBFAMILY NOT NAMED; Proteasome-interacting thioredoxin CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTTGTCCTCCTGCTTCTTGATCTCCGCC
Internal bar code:CATCCCAGAGTCGCATGCACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:707
LEAP-Seq percent confirming:67.5906
LEAP-Seq n confirming:2463
LEAP-Seq n nonconfirming:1181
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:TCGACCAGCTTTAATCCCAC
Suggested primer 2:GTAGGCTTCTCTCCCTCGCT