| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.060985 |
| Chromosome: | chromosome 2 |
| Location: | 5588424 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g109050 | OPR5 | OctotricoPeptide Repeat protein 5 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCATAGCCTGCCTCCCGCCCTCCTTTCCG |
| Internal bar code: | CAATCGATAGTCATCTATCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 803 |
| LEAP-Seq percent confirming: | 15.5391 |
| LEAP-Seq n confirming: | 147 |
| LEAP-Seq n nonconfirming: | 799 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTCTTTGTCGCTTTGCCT |
| Suggested primer 2: | AAATCCACCGCTACGTGAAC |