| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.060991 |
| Chromosome: | chromosome 3 |
| Location: | 9217112 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g213649 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCCTCATTTGGGGGGACACGAGCTGTGC |
| Internal bar code: | TGCACGTGAGTGATATGAACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 379 |
| LEAP-Seq percent confirming: | 98.2844 |
| LEAP-Seq n confirming: | 7963 |
| LEAP-Seq n nonconfirming: | 139 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGCGCAGCTGTGAGAGTG |
| Suggested primer 2: | GGGGTAGGGGCTGTTTTCTA |