| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.061008 |
| Chromosome: | chromosome 17 |
| Location: | 4339341 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g731100 | CPL14 | (1 of 8) PF10184 - Uncharacterized conserved protein (DUF2358) (DUF2358); Conserved in the Plant Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCCTCCTACCATCGTAGTTATTGGCCA |
| Internal bar code: | CTGGCGCAAAAAACAGATAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 830 |
| LEAP-Seq percent confirming: | 98.6548 |
| LEAP-Seq n confirming: | 3227 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGACTCAATACCTGGGGC |
| Suggested primer 2: | GGGAGCTACGCTGGTTAGTG |