Insertion junction: LMJ.RY0402.061018_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.061018
Chromosome:chromosome 7
Location:5589107
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g351825 (1 of 7) 2.7.10.2//2.7.12.1 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase // Dual-specificity kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTGTATGTTCTCCTGATGCCGCACGTAG
Internal bar code:CGGAGAAGCACCCGCGCAGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:899
LEAP-Seq percent confirming:99.6362
LEAP-Seq n confirming:3013
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GTGAAGCATTGAACCCCACT
Suggested primer 2:TAACCGGAACACACAAACGA