| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.061021 |
| Chromosome: | chromosome_17 |
| Location: | 2404375 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g715300 | PKD2 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCCTGTAGGCTCGGGTGTGAGGGTTTGGG |
| Internal bar code: | TGGGGCCCTCGCCCAGGTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 472 |
| LEAP-Seq percent confirming: | 99.8396 |
| LEAP-Seq n confirming: | 1245 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGAGTGCTGTATGAGCAG |
| Suggested primer 2: | CTGCAGGGACAAAAAGAAGC |