Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.061034 |
Chromosome: | chromosome 13 |
Location: | 4709073 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g604550 | (1 of 8) PF14580 - Leucine-rich repeat (LRR_9) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGGGGGGGGGGGGGCAGGGGGGGGCAGC |
Internal bar code: | GGGGTTCGCATTAACCCTATAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 107 |
LEAP-Seq percent confirming: | 33.1533 |
LEAP-Seq n confirming: | 4911 |
LEAP-Seq n nonconfirming: | 9902 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGTCGGTCTCAATCGTTGTT |
Suggested primer 2: | ATCATCGGGAGTGGAGATTG |