Insertion junction: LMJ.RY0402.061034_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.061034
Chromosome:chromosome 13
Location:4709073
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g604550 (1 of 8) PF14580 - Leucine-rich repeat (LRR_9) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGGGGGGGGGGGGGCAGGGGGGGGCAGC
Internal bar code:GGGGTTCGCATTAACCCTATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:107
LEAP-Seq percent confirming:33.1533
LEAP-Seq n confirming:4911
LEAP-Seq n nonconfirming:9902
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GGTCGGTCTCAATCGTTGTT
Suggested primer 2:ATCATCGGGAGTGGAGATTG