| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.061034 |
| Chromosome: | chromosome 13 |
| Location: | 4709097 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g604550 | (1 of 8) PF14580 - Leucine-rich repeat (LRR_9) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCGATGGCCAGCCTCTGCAGCGCGCTGA |
| Internal bar code: | ATACCGTATCTTAATCCGTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 824 |
| LEAP-Seq percent confirming: | 93.8654 |
| LEAP-Seq n confirming: | 67233 |
| LEAP-Seq n nonconfirming: | 4394 |
| LEAP-Seq n unique pos: | 140 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCGGTCTCAATCGTTGTT |
| Suggested primer 2: | ATCATCGGGAGTGGAGATTG |