| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.061052 |
| Chromosome: | chromosome 11 |
| Location: | 474904 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467594 | (1 of 18) PTHR10774 - EXTENDED SYNAPTOTAGMIN-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTGAGAAAGGAATGTGACACACATGGTC |
| Internal bar code: | CGAGGATGGAGGGGTACTGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 554 |
| LEAP-Seq percent confirming: | 99.7029 |
| LEAP-Seq n confirming: | 5034 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTCAAGTCAAATGACAA |
| Suggested primer 2: | TCTGTGAAGTGTGGCTTTCG |