Insertion junction: LMJ.RY0402.061062_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.061062
Chromosome:chromosome 6
Location:8577230
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g308650 (1 of 1) IPR003072//IPR003980//IPR005404 - Orphan nuclear receptor, NOR1 type // Histamine H3 receptor // Potassium channel, voltage dependent, Kv3.3 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTACCCGCGCCCTTCCACCGCATCGCGC
Internal bar code:TCCCAAGGTTGGACAAACTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:672
LEAP-Seq percent confirming:99.8171
LEAP-Seq n confirming:6002
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CACACCTCACACCACACCTC
Suggested primer 2:CGTTTTGTGGGAACTCCTGT