| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.061064 |
| Chromosome: | chromosome 3 |
| Location: | 2397894 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g159000 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCATCGCGCCAGGCGGAAACCACGAAAC |
| Internal bar code: | GCCGGCTGTGTTGCGCTTTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 477 |
| LEAP-Seq percent confirming: | 87.4199 |
| LEAP-Seq n confirming: | 1911 |
| LEAP-Seq n nonconfirming: | 275 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACCAAACAGCCCGTAGTG |
| Suggested primer 2: | GTTCCTCTTAACGCAGCACC |