| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.061086 |
| Chromosome: | chromosome 12 |
| Location: | 6444790 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g538000 | EZY5 | Early zygote expressed protein; (1 of 26) IPR001202 - WW domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGTGACGTGACGGCGCAGATTGGCCTTG |
| Internal bar code: | GGGGTGTCTCCGGTCACCGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 863 |
| LEAP-Seq percent confirming: | 98.7747 |
| LEAP-Seq n confirming: | 1451 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGTTGTTGGAGAGCAGAT |
| Suggested primer 2: | ATGGAAGACTTCTGAGCGGA |