| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.061094 |
| Chromosome: | chromosome 15 |
| Location: | 772895 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g640101 | (1 of 3) IPR000104//IPR006553 - Antifreeze protein, type I // Leucine-rich repeat, cysteine-containing subtype | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAGAACTCCACTTTACTCTTCAGACTTC |
| Internal bar code: | TGATGCAAAAGGTGTAAGTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 276 |
| LEAP-Seq percent confirming: | 41.1479 |
| LEAP-Seq n confirming: | 846 |
| LEAP-Seq n nonconfirming: | 1210 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGCCAACTGGTGAGTA |
| Suggested primer 2: | TACTCATGCCCACAAGCAAG |