| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.061124 |
| Chromosome: | chromosome 12 |
| Location: | 3324651 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497700 | SNE8 | (1 of 8) PTHR10366:SF411 - HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 173 PROTEIN; Cinnamoyl-CoA reductase/flavanone 4-reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACACACCGGCCTCACCTCAATCATGTCTC |
| Internal bar code: | GGTAGTTTTGTGATCGATCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 207 |
| LEAP-Seq percent confirming: | 91.8831 |
| LEAP-Seq n confirming: | 1981 |
| LEAP-Seq n nonconfirming: | 175 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGCAAGGTGCAGTGTACG |
| Suggested primer 2: | CAGAATAGCCGCAAGTGACA |