| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.061139 |
| Chromosome: | chromosome 2 |
| Location: | 3821193 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095550 | (1 of 1) PTHR14187//PTHR14187:SF51//PTHR22908 - ALPHA KINASE/ELONGATION FACTOR 2 KINASE // SUBFAMILY NOT NAMED // MIDASIN-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCCCGCCCCTGGAGCCCCTCCACCGGC |
| Internal bar code: | CCCCAGCCCGGATACTTCCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 291 |
| LEAP-Seq percent confirming: | 98.6413 |
| LEAP-Seq n confirming: | 363 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGGAGCTGAGGTTGAAGC |
| Suggested primer 2: | AAAGCTGCAATTATGGACCG |