| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.061151 |
| Chromosome: | chromosome 1 |
| Location: | 7197573 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g051700 | (1 of 6) PTHR24114//PTHR24114:SF2 - FAMILY NOT NAMED // LEUCINE-RICH REPEAT-CONTAINING PROTEIN 74 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGTGAGGGCCGCCGAAGTTGGGGTCCGT |
| Internal bar code: | ATCAGTGAATGGAACAAGTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 778 |
| LEAP-Seq percent confirming: | 98.9283 |
| LEAP-Seq n confirming: | 1477 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGGGTAGCAAAAATGCAC |
| Suggested primer 2: | CGTTAGCAGGCAGAAAGGAC |