Insertion junction: LMJ.RY0402.061161_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.061161
Chromosome:chromosome 9
Location:2015168
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g394400 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCACCACCTCGCGTCCGTCGCTGCTCCG
Internal bar code:GGGGGTCGCATGGCCAGTGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:313
LEAP-Seq percent confirming:68.9401
LEAP-Seq n confirming:3174
LEAP-Seq n nonconfirming:1430
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGAAGCGAACTACCTCGG
Suggested primer 2:GTTGTGGTGCTGTTGCTGTT