| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.061165 |
| Chromosome: | chromosome 6 |
| Location: | 2428733 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g268501 | (1 of 3) PF13563 - 2'-5' RNA ligase superfamily (2_5_RNA_ligase2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGAGCACATAACCGTCTGCTCCTTCAAA |
| Internal bar code: | GGCGGACGACGTCCTGAGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 336 |
| LEAP-Seq percent confirming: | 99.4475 |
| LEAP-Seq n confirming: | 180 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATACCACCAAAACCTGCG |
| Suggested primer 2: | CGATCCTGCCTAGCTTTACG |