| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.061179 |
| Chromosome: | chromosome 4 |
| Location: | 2294409 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g220350 | ATPVA3 | Vacuolar ATP synthase subunit A; (1 of 3) K02154 - V-type H+-transporting ATPase subunit a (ATPeV0A, ATP6N) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTATGACGGAGGTATGTACGGAGGCATCGC |
| Internal bar code: | GGTTCAAAAGGGACCTGAGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1088 |
| LEAP-Seq percent confirming: | 99.506 |
| LEAP-Seq n confirming: | 7453 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTTTGGTGAACGGTTG |
| Suggested primer 2: | TGCCACGGTCACATAGTCAT |