| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.061185 |
| Chromosome: | chromosome 2 |
| Location: | 5956421 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g111950 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGAGGTGGCTGCCTTACCCACCGAGCTT |
| Internal bar code: | ATGGCGGCGGGGGCGAACGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 926 |
| LEAP-Seq percent confirming: | 99.8398 |
| LEAP-Seq n confirming: | 3739 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCGCATCTCAACTACGGT |
| Suggested primer 2: | AGACAGCTGGCAACTGGAAT |