| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.061211 |
| Chromosome: | chromosome 3 |
| Location: | 6491663 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g195000 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACTTGGCCTGACGGCAACCGTGCCTGT |
| Internal bar code: | GTGACGGTGGTGAGATGCGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 960 |
| LEAP-Seq percent confirming: | 98.9699 |
| LEAP-Seq n confirming: | 5957 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGTTGTCGAACGTGAGCG |
| Suggested primer 2: | GCAATGCAGTGGCTATGCTA |