| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.061287 |
| Chromosome: | chromosome 12 |
| Location: | 9374589 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g540351 | SELENOW2,SELW2 | Selenoprotein W2; (1 of 3) IPR011893//IPR012336 - Selenoprotein, Rdx type // Thioredoxin-like fold | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGATGGCTCAGACCTAAGGAGTTGCGAA |
| Internal bar code: | ACCGTGGAAGGAGCTGGTGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 972 |
| LEAP-Seq percent confirming: | 97.4812 |
| LEAP-Seq n confirming: | 1819 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACATCCGCTCCAGCTCTC |
| Suggested primer 2: | CCAACGCCTCTGATCTCTTC |