Insertion junction: LMJ.RY0402.061288_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.061288
Chromosome:chromosome 9
Location:2522513
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g391000 GT90F14,GT90-14 (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 14 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCGTAAGCACGCTGCTAGCTGCACCTGC
Internal bar code:TAGAACCAAAAATACACCAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1031
LEAP-Seq percent confirming:99.5403
LEAP-Seq n confirming:6280
LEAP-Seq n nonconfirming:29
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GCCATACTCGCCTAAACAGC
Suggested primer 2:CCATAGCCACTAGCCAGAGC