| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.061310 |
| Chromosome: | chromosome 6 |
| Location: | 5093277 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280850 | PBG1 | 20S proteasome beta subunit, type 4; (1 of 1) K02736 - 20S proteasome subunit beta 7 (PSMB4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAGTGGCAGGGCCGCCTGGAGCAGATTG |
| Internal bar code: | AGACCAAGATGGCTATCTGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 470 |
| LEAP-Seq percent confirming: | 99.2679 |
| LEAP-Seq n confirming: | 2305 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTTCACAGCACGTTCAGA |
| Suggested primer 2: | CGTCCACATCACCACACTTC |