| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.061323 |
| Chromosome: | chromosome 9 |
| Location: | 3453965 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389356 | (1 of 1) K03457 - nucleobase:cation symporter-1, NCS1 family (TC.NCS1) | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGGGGAGCGGGCGCGGAGACGCGGCCC |
| Internal bar code: | AATCTGTAGCTCGGCCGGCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 620 |
| LEAP-Seq percent confirming: | 99.647 |
| LEAP-Seq n confirming: | 18916 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCCTCCAACCCTTTCTCC |
| Suggested primer 2: | CACCTTATGCCGTCATGTTG |