| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.061331 |
| Chromosome: | chromosome 16 |
| Location: | 6359087 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674050 | ADH12 | Alcohol dehydrogenase; (1 of 1) 1.6.5.5 - NADPH:quinone reductase / Zeta-crystallin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGGCCAGGGCAAGCTGCGCGTGGAGGT |
| Internal bar code: | TCGTCTTAGTTATATAACGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 863 |
| LEAP-Seq percent confirming: | 92.5694 |
| LEAP-Seq n confirming: | 1034 |
| LEAP-Seq n nonconfirming: | 83 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAAATAGCTGTCGCTGTG |
| Suggested primer 2: | CTTGCCCTGCACACAATCTA |