| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.061366 |
| Chromosome: | chromosome 7 |
| Location: | 5480892 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g350867 | (1 of 37) PF13639 - Ring finger domain (zf-RING_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCATATGGTACAGGCACACGCACACGCC |
| Internal bar code: | TTATTGAGTTTCGGAGAATCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 166 |
| LEAP-Seq percent confirming: | 87.8545 |
| LEAP-Seq n confirming: | 1208 |
| LEAP-Seq n nonconfirming: | 167 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATCCACAACATGATCAGC |
| Suggested primer 2: | TACTCCGATGGATACTGGGG |